Effects of epinephrine on angiogenesis-related gene expressions in cultured rat cardiomyocytes

作     者：Henry Liu Lisa Sangkum Geoffrey Liu Michael Green Marilyn Li Alan Kaye 

作者机构：Department of Anesthesiology & Perioperative MedicineDrexel University College of MedicineHahnemann University Hospital Division of Genomic DiagnosticsDept.of Pathology & Laboratory MedicineChildren's Hospital of PhiladelphiaPerelman School of Medicine University of Pennsylvania Department of AnesthesiologyLSUHSC-New Orleans 

出 版 物：《The Journal of Biomedical Research》 (生物医学研究杂志（英文版）)

年 卷 期：2016年第30卷第5期

页      面：380-385页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by internal funding from the Department of Anesthesiology and Perioperative Medicine 

主　　题：epinephrine angiogenesis gene expression cardiomyocytes angiopoietin-2 neuregulin 1 plasminogen activator inhibitor-1 SPARC-related modular calcium-binding protein 

摘      要：Epinephrine is often used for the treatment of patients with heart failure, low cardiac output and cardiac arrest. It can acutely improve hemodynamic parameters; however, it does not seem to improve longer term clinical outcomes. Therefore, we hypothesized that epinephrine may induce unfavorable changes in gene expression of cardiomyocyte. Thus, we investigated effects of epinephrine exposure on the mediation or modulation of gene expression of cultured cardiomyocytes at a genome-wide scale. Our investigation revealed that exposure of cardiomyocytes to epinephrine in an in vitro environment can up-regulate the expression ofangiopoietin-2 gene （~ 2.1 times）, and down-regulate the gene expression of neuregulin 1 （-3.7 times）, plasminogen activator inhibitor-1 （-2.4 times） and SPARC-related modular calcium-binding protein-2 （-4.5 times）. These changes suggest that epinephrine exposure may induce inhibition of angiogenesis-related gene expressions in cultured rat cardiomyocytes. The precise clinical significance of these changes in gene expression, which was induced by epinephrine exposure, warrants further experimental and clinical investigations.