In vitro Inhibitory Activity of Shisandra chinensis and Polygonatum sibiricum against Vibrio harveyi and Its Biofilms

作     者：Zhifeng HUANG Huanying PANG Yang HUANG Yucong HUANG Yishan LU Jichang JIAN 

作者机构：College of FisheriesGuangdong Ocean University/Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals/Key Laboratory of Diseases Controlling for Aquatic Economic Animals of Guangdong Higher Education InstitutionsZhanjiang 524088China 

出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)

年 卷 期：2016年第5卷第4期

页      面：57-60页

学科分类：0906[农学-兽医学] 09[农学] 

基　　金：Supported by Project of Enhancing School with Innovation of Guangdong Ocean University(GDOU2015050216) Major Program for the Fundamental Research of the Department of Education of Guangdong Province(2014GKXM046) International Cooperation Innovation Platform Project of Universities in Guangdong Province(2013gjhz0008) 

主　　题：Shisandra chinensis Polygonatum sibiricum Vibrio harveyi In vitro inhibitory activity Biofilm 

摘      要：[Objective] This study aimed to analyze the in vitro inhibitory activity of Shisandra chinensis and Polygonatum sibiricum against Vibrio harveyi and its biofilms. [Result] By agar diffusion test, in vitro inhibitory activity of 5. chinensis and P. sibiricum against V. harveyi was investigated. The minimal inhibitory concentration （ MIC） and minimal bactericidal concentration （MBC） of 5. chinensis and P. sibiricum against V. harveyi were determined by doubling dilution meth-od. The inhibitory activity of 5. chinensis and P. sibiricum on the formation of V. harveyi biofilms was evaluated by modified MTT assay. [ Result ] Both 5. chinen-sis and P. sibiricum had inhibitory activity against V. harveyi. The inhibition zone diameter of 5. chinensis against V. harveyi was 17. 95 mm; MIC and MBC of 5. chinensis were both 3.125 mg/ml. The inhibition zone diameter of P. sibiricum against V. harveyi was 12. 22 mm; MIC and MBC of P. sibiricum were 3.125 and 6.250 mg/ml, respectively. When the concentration was higher than 6. 25 mg/ml, 5. chinensis decoction had extremely significant inhibitory activity against V. harveyi （P 〈 0. 01） ; when the concentration was higher than 3. 125 mg/ml, P. sibiricum had extremely significant inhibitory activity against V. harveyi （P 〈0. 01）. [ Conclusion] 5. chinensis and P. sibiricum could significantly inhibit V. harveyi and its biofilms.