Enhancement of radiosensitivity of MCF-7 breast cancer cells subjected to X-ray or carbon-ion irradiations

作     者：Yi Xie Bing Wang Kaoru Tanaka Hong-Yan Li Qiu-Yue Zhao Hong Zhang 

作者机构：Institute of Modern PhysicsChinese Academy of Sciences509 Nanchang RoadLanzhou 730000China Key Laboratory of Heavy Ion Radiation Biology andMedicine of Chinese Academy of SciencesLanzhouChina Key Laboratory of Heavy Ion Radiation Medicine of GansuProvinceLanzhouChina National Institute of Radiological SciencesChibaJapan Graduate School of University of Chinese Academy of SciencesBeijing 100039China 

出 版 物：《Nuclear Science and Techniques》 (核技术（英文）)

年 卷 期：2016年第27卷第1期

页      面：75-81页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the National Natural Science Foundation of China(U1432248) Gansu Provincial Science and Technology Department(0702NKDA045) Japan Society for the Promotion of Science and National Institute of Radiological Sciences of Japan(11J364) 

主　　题：MCF-7细胞 X射线照射 放射治疗 碳离子 乳腺癌 增敏作用 人端粒酶逆转录酶 细胞周期阻滞 

摘      要：The sensitivity of cancer cells to radiation therapy varies based on cell cycle phase. Here we evaluated the differences between X-ray and carbon-ion irradiation with respect to cellular radiosensitivity and cancer cycle arrest in the breast cancer cell line, MCF-7. The cell survival rate, cell cycle distribution and the presence of apoptosis were measured by clonogenic assay and flow cytometry. BRCA1 and p21 protein levels were analyzed by Western blot, and the levels of human telomerase reverse transcriptase(h TERT) m RNA expression and telomere length were detected with real-time polymerase chain reaction. The results show a significant dose-dependent effects on survival rate, apoptosis and protein levels in the carbon-ion group of MCF-7 cells. Decreased proliferation was not observed at 2 Gy X-ray irradiation. There were significant differences in cellular cycle arrest, apoptosis percentages and BRCA1 and p21 protein expression between X-ray and heavy-ion groups. The results indicatedthat increasing in BRCA1 and p21 expression, and attenuation of h TERT gene expression induced by heavy-ion irradiation in MCF-7 cells might relate to mechanism of cellular radiosensitivity in G2/M arrested phase.