Characteristics of Ca2+-exocytosis coupling in isolated mouse pancreatic β cells

作     者：Qian GE~(2,4) Yong-ming DONG~(2,4) Zhi-tao HU~2 Zheng-xing WU~(2,5) Tao XU~(2,3,5)2 College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China 3 National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China 

作者机构：College of Life Science and Technology Huazhong University of Science and Technology Wuhan China National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2006年第27卷第7期

页      面：933-938页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：国家自然科学基金 国家863计划 

主　　题：mouse pancreatic βcells Ca2+-exocytosis coupling 

摘      要：Aim:To characterize Ca2+-stimulated exocytosis in isolated mouse pancreatic β***:An improved method was described for isolation of mouse pan-creatic β cells by collagenase *** Ca2+channel current and the membranecapacitance were examined by using the whole-cell patch clamp ***:Using depolarization and flash photolysis of caged Ca2+toinduce Ca2+-dependent exocytosis in β cell from KM mouse,we have explored thecharacteristics of the Ca2+channel current and the relationship between Ca2+signals and *** averaged peak Ca2+current measured at +20 mV was-60±6 pA（n=13）.Conclusion:We characterized three kinetically different pools ofvesicles in mouse pancreatic β cells,namely an immediately releasable pool,areadily releasable pool,and a reserve pool.