Studies on the Correlation between Germination and cAMP Content of Different Color Chlamydospores in Ustiloginoidea virens

作     者：Xiaojuan LI Yuan LI Zuohua REN Lang YANG Juanfang CHEN Dandan HONG Shuangqing LIU Xinyu ZHOU Minjie LIU Erming LIU 

作者机构：College of Plant protectionHunan Agricultural UniversityChangsha 410128China Institute of Plant ProtectionChangsha 410125China College of Information Science and TechnologyHunan Agricultural UniversityChangsha 410128China 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2016年第17卷第3期

页      面：694-699页

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 0901[农学-作物学] 090402[农学-农业昆虫与害虫防治] 090102[农学-作物遗传育种] 

基　　金：supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China(Class tutors,Grant No.20114320110008) the Scientific Research Fund of Hunan Provincial Education Department,China(Grant No.11A052,No.12JJ4028) Inquiry Learning and Innovative Experiment Projects of Hunan Provincial Undergraduate(SCX1219) 

主　　题：Ustiloginoidea virens Chlamydospore Germination rate c AMP 

摘      要：Ustilaginoidea virens is the causal agent of a serious disease of rice. To reveal the relationship between germination and the 3 -5 -cyclic adenosine monophosphate(c AMP) content from the dormant(black or green-black) and non-dormant(yellow)chlamydospore in Ustiloginoidea virens,this study adopts ultrasonic-bath method and high-performance liquid chromatography(HPLC) method, for extraction c AMP content of the different color chlamydospore. The results demonstrated that, as for the freshly chlamydospores collected from the false smut balls during their germination, c AMP content of yellow chlamydospore appeared a slight growth during 0-12 h, and showed a rapid declining as the germination time extended(12-48 h). Above all, in yellow chlamydospores, the germination rate and the content of c AMP presented a very notably negative correlation(|r|=0.861 9r0.01=0.834), but the correlation between germination rate and content of c AMP was not obvious in black chlamydospores. The germination rate and the content of c AMP presented a notably positive correlation under different storage period(0, 2, 4, 6, 8,10 months) of the two color chlamydospores(the yellow of chlamydospore: |r | = 0.785 1 r0.05= 0.707; the black of chlamydospore: |r| = 0.957 9 r0.05= 0.707).