Isolation and characterization of the murine Nanog gene promoter
Isolation and characterization of the murine Nanog gene promoter作者机构：Laboratory of Molecular Cell Biology Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China
出 版 物：《Cell Research》 (细胞研究(英文版))
年 卷 期：2005年第15卷第5期
页 面：317-324页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学]
主 题：Nanog promoter F9 EC cells Oct-1 Oct-4 Oct-6.
摘 要：Nanog protein is expressed in the interior cells of compacted morulae and maintained till epiblasts but downregulated by implantation stage. It is also expressed in embryonic stem cells, embryonic carcinoma cells and embryonic germ cells but disappeared in differentiated ES cells. In this study, we have isolated, sequenced, and performed the first characterization of the Nanog promoter. The transcription start sites were mapped by primer extension analysis. Two promoter regions were found upstream the transcription start sites and the expression of major Nanog promoter/ reporter gene construct is abolished in differentiated F9 EC cells as compared to the undifferentiated counterpart. We also showed that a putative octamer motif (ATGCAAAA) is necessary for the major promoter activity. Gel shift and supershift assays showed that Oct-1, Oct-4 and Oct-6 protein selectively bind to the octamer motif.
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