GABAergic Interneurons are Required for Generation of Slow CA1 Oscillation in Rat Hippocampus

作     者：Yuan Xu Lidan Wang Yu-zhang Liu Yan Yang Xiaolin Xue Zhiru Wang 无

作者机构：Institute and Key Laboratory of Brain Functional Genomics of The Ministry of Education of ChinaShanghai Key Laboratory of Brain Functional GenomicsSchool of Life Sciences East China Normal University 

出 版 物：《Neuroscience Bulletin》 (神经科学通报（英文版）)

年 卷 期：2016年第32卷第4期

页      面：363-373页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 07[理学] 1001[医学-基础医学(可授医学、理学学位)] 071006[理学-神经生物学] 

基　　金：supported by grants from the National Natural Science Foundation of China(91132711 30970960 31471078) a Key Scientific Project of the Shanghai Science and Technology Commission,China(15JC1400102) the Shanghai Pu-Jiang Program,China(08PJ14044) 

主　　题：Hippocampus - Oscillation SynchronizationGABAergic interneuron GABAA receptor 

摘      要：Neuronal oscillations are fundamental to hip- pocampal function. It has been shown that GABAergic interneurons make an important contribution to hippocampal oscillations, but the underlying mechanism is not well understood. Here, using whole-cell recording in the complete hippocampal formation isolated from rats at postnatal days 14-18, we showed that GABAA receptormediated activity enhanced the generation of slow CA1 oscillations. In vitro, slow oscillations （0.5-1.5 Hz） were generated in CA1 neurons, and they consisted primarily of excitatory rather than inhibitory membrane-potential changes. These oscillations were greatly reduced by blocking GABAA receptor-mediated activity with bicuculline and were enhanced by increasing such activity with midazolam, suggesting that interneurons are required for oscillation generation. Consistently, CA1 fast-spiking interneurons were found to generate action potentials usually preceding those in CA1 pyramidal cells. These findings indicate a GABAA receptor-based mechanism for the generation of the slow CA1 oscillation in the hippocampus.