Association of H-FABP gene polymorphisms with intramuscular fat content in Threeyellow chickens and Hetian-black chickens

作     者：Yong Wang Xiaohong Hui Huie Wang Tursunjan Kurban Chao Hang Ying Chen Jinming Xing Jiufeng Wang 

作者机构：College of Veterinary MedicineChina Agricultural University Key Laboratory of Tarim Animal Husbandry Science & Technology of Xinjiang Production and Construction Groups/College of Animal ScienceTarim University 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2016年第7卷第3期

页      面：366-373页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0703[理学-化学] 0836[工学-生物工程] 

基　　金：supported by the National Natural Science Foundation of China(Project No.31160521) Tarim University Principal Fund(Project No.TDZKGG201504) the project of Key Laboratory of Tarim Animal Husbandry Science and Technology,Xinjiang Production&Construction Group(Project No.HS201302) 

主　　题：Hetian black Chicken H FABP m RNA Expression Polymorphism Three yel ow Chicken 

摘      要：Background： To explore the relationship between the heart-type fatty acid binding protein（H-FABP） gene and intramuscular fat（IMF）, a polymorphism of the second exon of the H-FABP gene was investigated in 60 Three-yellow chickens（TYCs） and 60 Hetian-black chickens（HTBCs）.Results： The IMF contents of the cardiac, chest and leg muscles in HTBC were increased compared with TYC. Both TYC and HTBC populations exhibited Hardy-Weinberg Equilibrium（HWE） according to the χ2test. Three variations of the two birds were detected, namely, G939 A, G982 A and C1014 T. HTBCs with the TT genotypes exhibit increased IMF content in the chest muscles compared with the TC genotype. Thus, the G982 A site could be considered a genetic marker for selecting increased IMF content in the chest muscles of HTBC. The correlation coefficients revealed that H-FABP m RNA expression was negatively correlated with the IMF content in the cardiac, chest and leg muscles of HTBC and in the cardiac and chest muscles of TYC. The relative m RNA expression of H-FABP was reduced in the cardiac and leg muscles of HTBC compared with TYC, but this difference was not observed at the protein level, as assessed by Western blot ***： These findings offer essential data that can be useful in the breeding program of HTBC and future research exploring the role of H-FABP in IMF deposition and regulation in chickens.