Localization of Banana bunchy top virus and cellular compartments in gut and salivary gland tissues of the aphid vector Pentalonia nigronervosa
Localization of Banana bunchy top virus and cellular compartments in gut and salivary gland tissues of the aphid vector Pentalonia nigronervosa作者机构：Department of Plant and Environmental Protection Sciences University of Hawaii 3050 Maile Way Gilmore Hall 96822 Honolulu HI Department of Molecular Biosciences and Bioengineering University of Hawafi 1955 East-West Road Honolulu HI USA
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2016年第23卷第4期
页 面：591-602页
核心收录：
学科分类：0906[农学-兽医学] 09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治]
基 金：supported by the University of Hawaii Hatch fund
主 题：aphid digestive system aphid salivary glands cellular markers circulative virus immunofluorescence transcytosis
摘 要：Banana bunchy top virus （BBTV） （Nanoviridae： Babuvirus） is transmitted by aphids of the genus Pentalonia in a circulative manner. The cellular mechanisms by which BBTV translocates from the anterior midgut to the salivary gland epithelial tissues are not understood. Here, we used multiple fluorescent markers to study the distribution and the cellular localization of early and late endosomes, macropinosomes, lysosomes, microtubules, actin filaments, and lipid raft subdomains in the gut and principal salivary glands ofPentalonia nigronervosa. We applied colabeling assays, to colocalize BBTV viral particles with these cellular compartments and structures. Our results suggest that multiple potential cellular processes, including clathrin- and caveolae-mediated endocytosis and lipid rafts, may not be involved in BBTV internalization.
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