OPG inhibit the differentiation of osteoclasts through DOCK5 signal pathway
OPG inhibit the differentiation of osteoclasts through DOCK5 signal pathway作者机构：College of Veterinary Medicine Yangzhou University Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses
出 版 物：《畜牧与饲料科学》 (Animal Husbandry and Feed Science)
年 卷 期：2015年第Z1期
页 面：333-333页
学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学]
主 题：DOCK5 osteoclast differentiation lamellipodium osteoprotegerin
摘 要：Rac1 is activated by DOCK5 which affect actin polymerization through PAK1,LIMK and *** is confirmed that OPG(osteoprotegerin,OPG) have an effect on differentiation of osteoclasts,while its mechanism is not yet *** research,with transmission electron microscope,real-time quantitative PCR and western blot,found that lamellipodium of osteoclast precursor cells was increased abnormally,transcription level of DOCK5 was decreased significantly,while PAK1,PAK2,PAK3,PAK1/2/3,LIMK2 and Cofilin protein expression and phosphorylation level of PA K1 Ser199/204 and PAK2 Serl92/197 sites had no change after treatment of 100 ng/mL *** all this,phosphorylation of Ser144 /Thr423 in PAK1,Ser141/Thr402 in PAK2,Thr508 in LIMK1 and Thr505 in LIMK2 was decreased,however that of Cofilin was *** results indicate that OPG down regulate DOCK5 transcription,affect Rac1 activation,and decrease phosphorylation of Ser144 /Thr423 of PAK1 and Serl41/Thr402 of *** then decrease phosphorylation of Thr508 in LIMKl and Thr505 in LIMK2,increase Cofilin phosphorylation,shear on the ends of actin filament,remove the cap structure and promote the formation of new actin *** a result,it increases the number of lamellipodium abnormally and inhibits osteoclast differentiation.
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