Rab27b is Involved in Lysosomal Exocytosis and Proteolipid Protein Trafficking in Oligodendrocytes

作     者：Yun-Tian Shen Yun Gu Wen-Feng Su Jing-fei Zhong Zi-Han Jin Xiao-Song Gu Gang Chen 无

作者机构：Jiangsu Key Laboratory of NeuroregenerationCo-innovation Center of NeuroregenerationNantong University Xinglin CollegeNantong University 

出 版 物：《Neuroscience Bulletin》 (神经科学通报（英文版）)

年 卷 期：2016年第32卷第4期

页      面：331-340页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 07[理学] 1001[医学-基础医学(可授医学、理学学位)] 071006[理学-神经生物学] 

基　　金：supported by the National Natural Science Foundation of China(31071251 and 81471255) the National Basic Research Development Program of China(2014CB542202) the Basic Research Program of the Department of Education,Jiangsu Province,China(14KJA310004) a Natural Science Research Project of Nantong Science and Technology Bureau,Jiangsu Province,China(HS2013014) the Natural Science Research Project of Nantong University,Jiangsu Province,China(12Z045,13Z008) 

主　　题：Myelination Neuron-glial interaction Oligodendrocyte Lysosome Rab27b 

摘      要：Myelination by oligodendrocytes in the central nervous system requires coordinated exocytosis and endocytosis of the major myelin protein, proteolipid protein （PLP）. Here, we demonstrated that a small GTPase, Rab27b, is involved in PLP trafficking in oligodendrocytes. We showed that PLP co-localized with Rab27b in late endosomes/lysosomes in oligodendrocytes. Short hairpin- mediated knockdown of Rab27b not only reduced lysosomal exocytosis but also greatly diminished the surface expression of PLP in oligodendrocytes. In addition, knockdown of Rab27b reduced the myelin-like membranes induced by co-culture of oligodendrocytes and neurons. Our data suggest that Rab27b is involved in myelin biogenesis by regulating PLP transport from late endosomes/ lysosomes to the cell membrane in oligodendrocytes.