Numerical Simulation of Mixing in a Micro-well Scale Bioreactor by Computational Fluid Dynamics

作     者：Hu Zhang Sally Lamping Parviz Ayazi Shamlou 

作者机构：Advanced Biochemical Engineering CentreUniversity College LondonTorrington PlaceLondonWC1E 7JE 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究(英文版))

年 卷 期：2002年第18卷第2期

页      面：113-116页

核心收录：

学科分类：07[理学] 0703[理学-化学] 

基　　金：SupportedbyUCLORSAwardandKCWongScholarship 

主　　题：CFD Numerical simulation Fermenter Snapshot method Two fluid Population balance 

摘      要：The introduction of the multi well plate miniaturisation technology with its associated automated dispensers, readers and integrated systems coupled with advances in life sciences has a propelling effect on the rate at which new potential drug molecules are discovered. The translation of these discoveries to real outcome now demands parallel approaches which allow large numbers of process options to be rapidly assessed. The engineering challenges in achieving this provide the motivation for the proposed work. In this work we used computational fluid dynamics(CFD) analysis to study flow conditions in a gas liquid contactor which has the potential to be used as a fermenter on a multi well format. The bioreactor had a working volume of 6 5 mL with the major dimensions equal to those of a single well of a 24 well plate. The 6 5 mL bioreactor was mechanically agitated and aerated by a single sparger placed beneath the bottom impeller. Detailed numerical procedure for solving the governing flow equations is given. The CFD results are combined with population balance equations to establish the size of the bubbles and their distribution in the bioreactor. Power curves with and without aeration are provided based on the simulated results.