Fabrication and characterization of gold nano particles for DNA biosensor applications

作     者：Ahmed Mishaal Mohammed 

作者机构：Chemistry Department College of Science Anbar University Institutes of Nano Electronic Engineering University Malaysia Perlis 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2016年第27卷第5期

页      面：801-806页

核心收录：

学科分类：07[理学] 08[工学] 080202[工学-机械电子工程] 070302[理学-分析化学] 0802[工学-机械工程] 0703[理学-化学] 

基　　金：supported by INEE at (Uni MAP),through the Nano Technology project therefore the Institute of Nano Electronic Engineering (INEE) at University Malaysia Perlis (Uni MAP) for supporting this work 

主　　题：Gold nanoparticlesElectrochemical DNA hybridization Dielectric analyzer 

摘      要：This research involves the preparation ofa biosensor using silicon oxide for biomedical applications, and its effective use for the detection of target DNA hybridization. An electrochemical DNA biosensor was successfully fabricated by using （3-aminopropyl） tri-ethoxysilane （APTES） as a linker molecule combined with gold nanoparticles （GNPs） on a thermally oxidized 5i02 thin film. The size of the GNPs was calculated by utilizing UV-vis data with an average calculated particle size within the range of 30 - 5 nm, and characterization by transmission electron microscopy （TEM） and atomic force microscopy （AFM）. The GNP-modified SiO2 thin films were electrically characterized through the measurement of capacitance, permittivity and conductivity using a low-cost dielectric analyzer. The capacitance, permittivity and conductivity profiles of the fabricated sensor clearly differentiated DNA immobilization and hybridization.