In silico and in vitro genotoxicity evaluation of descarboxyl levofloxacin,an impurity in levofloxacin
In silico and in vitro genotoxicity evaluation of descarboxyl levofloxacin,an impurity in levofloxacin作者机构：Shandong Institute for Food and Drug Control
出 版 物：《中国药理学与毒理学杂志》 (Chinese Journal of Pharmacology and Toxicology)
年 卷 期：2013年第3期
页 面：470-471页
核心收录：
学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学]
主 题：genotoxicity impurity descarboxyl levofloxacin SARs CHL chromosomal aberration
摘 要：OBJECTIVE To evaluate the genotoxicity of Descarboxyl Levofloxacin by In silico and In vitro *** Assessment of genotoxicity using Derek for Windows We used DEREK for Windows(DfW,Lhasa Ltd.,Leeds,UK) version 11.0 for toxicity *** structures are entered into the program in *** *** program applies structure-activity relationships 〔(Q) SARs〕and other expert knowledge rules to derive a reasoned prediction about the potential toxicity of the query *** reverse mutation assay(Ames test) The Salmonella histidine point mutation assay proposed by Maron and Ameswas *** *** histidine autotrophic strains(TA97,TA98,TA100 and TA102) used in this test were obtained from Shandong center for disease *** saline(NS) was used as a vehicle control.2-Aminofluorene(2-AF,10 μg /plate),1,8-dihydroxyanthraquinone(50 μg /plate),Sodium azide(NaN3,1.5 μg /plate),and Dexon(50 μg /plate) were used as positive *** levofloxacin was assayed at concentrations of 31.25,62.5,125,250,and 500 μg /***,0.1 ml of test substance(or control solution),0.1 ml of bacterial culture 〔(1-2) × 1012 L-1 〕and 0.5 ml of S9 mix(NS for treatment without activation) were mixed and poured onto the agar plates after addition of 1.8 ml of top agar(0.6% agar Difco,0.5% NaCl,50 μmol·L-1 L-histidine,50 μmol·L-1 biotin,45℃) at 45℃.Plates were inverted and incubated for 48h at 37℃ in the *** colonies were counted and the background lawn was inspected for signs of toxicity or compound *** assay was performed two times in triple and included negative and positive *** al aberrations in Chinese ham ster lung cells Chinese Hamster Lung(CHL) cells were obtained from Shandong center for disease *** cells were grown in DMEM medium supplemented with 10% heat-inactivated calf serum at 37℃ in an atmosphere containing 5% of *** test for Chromosomal aberrations in CHL cells was con
同方期刊数据库