Regulation of tissue-type plasminogen activator and plasminogen activator inhibitor type-1 in cultured rat Sertoli and Leydig cells

作     者：刘以训 杜群 周红明 刘奎 胡召元 

作者机构：State Key Laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100080 China State Key Laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100080 China Department of PathologyMcMaster UniversityHamiltonOntarioCanada 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：1996年第39卷第1期

页      面：37-44页

学科分类：0710[理学-生物学] 07[理学] 071002[理学-动物学] 

基　　金：Project supported by the National Natural Science Foundation of China and State Family Planning Commission 

主　　题：Sertoli cells Leydig cells tissue type plasminogen activator plasminogen activator inhibitor type-1. 

摘      要：New data are provided to show that (i) rat Sertoli cells produce two types of plasminogen activators, tissue type (tPA) and urokinase type (uPA), and a plasminogen activator inhibitor type-1 (PAI-1); (ii) both tPA (but not uPA) and PAI-1 secretion in the culture are modified by FSH, forskolin, dbcAMP, GnRH, PMA and growth factors (EGF and FGF), but not by hCG and androstenedione (△4); (iii) in vitro secretion of tPA and PA-PAI-1 complexes of Sertoli cells are greatly enhanced by presence of Leydig cells which produce negligible tPA but measurable PAI-1 activity;(iv) combination culture of Sertoli and Leydig cells remarkably increases FSH-induced PAI-1 activity and decreases hCG- and forskolin-induced inhibitor activity as compared with that of two cell types cultured alone. These data suggest that rat Sertoli cells, similar to ovarian granulosa cells, are capable of secreting both tPA and uPA, as well as PAI-1. The interaction of Sertoli cells and Leydig cells is essential for the cells to response to hormone stimulation for tPA and PAI-1 secretion.