Identification of Gardenoside and Sarsasapogenin from Qingwen Baidu Granules by TLC

作     者：Guo Shijin Wang Yanping Zhang Zhimei Fu shijun Xu Qianqian Zhang Ying Shen Zhiqiang 

作者机构：Shandong Binzhou Animal Science and Veterinary Medicine Academy Binzhou 256600 China Shandong Lvdu Ante Veterinary Drug Co. Ltd. Binzhou 256600 China 

出 版 物：《Animal Husbandry and Feed Science》 (动物与饲料科学（英文版）)

年 卷 期：2016年第8卷第2期

页      面：79-81页

学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：Supported by Technical Innovation Project of Shandong Province(201210916001) 

主　　题：Qingwen baidu granules Gardenoside Sarsasapogenin TLC identification 

摘      要：[ Objective ] We aimed to establish the identification method of gardenoside and sarsasapogenin in Qingwen baidu granules. [ Method] With ethyl ace- tate-acetone-formic acid-water （10：7：2：0.5） as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 100 ~C until the spots were clearly visible, and the existence of gardenoside was checked under natural light. With toluene-acetone（9：l ） as the developer and 5% vanillin sulfuric acid solution as the chromogenic reagent, the samples were heated at 105 ~C until the spots were clearly visible, and the existence of sarsasapogenin was checked under natural light. [Result] Qingwen baidu granules had the same spots with gardenoside and sarsasapogenin at the same Rfvalue under natural light. [ Conclusion] A TLC method detecting gardenoside and sarsasapogenin in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of gardenoside and sarsasapogenin.