Vaccination against Schistosoma japonicum Infection by DNA Vaccine Encoding Sj22.7 Antigen

作     者：Gan DAI Shiping WANG Junlong YU Shaorui XU Xianchu PENG Zhuo HE Xueqin LIU Songhua ZHOU and Fen LIU Institute of Schistosomiasis Research.Xiangya School of Medicine,Central South University,Changsha 410078 China 

作者机构：InstituteofSchistosomiasisResearchXiangyaSchoolofMedicineCentralSouthUniversityChangsha410078China 

出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))

年 卷 期：2007年第39卷第1期

页      面：27-36页

核心收录：

学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基　　金：ThisworkwassupportedbythegrantsfromtheChinaNational"TenthFive-YearPlan"ImportantSpecialProgram(2002AA2Z3343) theHi-TechResearchandDevelopmentProgramofChina(2004AA2Z3530,2004AA2Z3522) theHunanProvince"EleventhFiv 

主　　题：response Schistosoma japonicum DNA vaccine Sj22.7 gene expression immune protection immune 

摘      要：To observe the in vitro expression of DNA vaccine pcDNA3-Sj22.7 and its immunologicaleffect in mice,the recombinant plasmid pcDNA3-Sj22.7 was used to transfect HeLa cells with liposome-mediated method and the expression of Sj22.7 mRNA and protein was examined using reversetranscription-polymerase chain reaction, sodium dodecylsulfate-polyacrylamide gel electrophoresis andWestern ***,the ability of pcDNA3-Sj22.7 to protect against Schistosoma japonicum challengeinfections was analyzed according to worm reduction rate and egg reduction rate after vaccination of *** serum levels of specific IgG antibody and T lymphocyte proliferation response were also *** the challenge infection,Sj22.7-driven interferon (IFN)-γand interleukin (IL)-4 was also *** showed that pcDNA3-Sj22.7 could express Sj22.7 mRNA and protein in *** in a worm reduction rate of 29.70%,egg reduction rate of 47.25% (liver) and 51.73% (intestine),and egg reduction rate of 25.90% (eggs per female),suggesting induction of significant anti-fecundityin the pcDNA3-Sj22.7 group. Enzyme-linked immunosorbent assay and Western blot analysis indicatedthat immunized mice generated specific IgG against Sj22.7.T lymphocytes from mice immunized withpcDNA3-Sj22.7 showed a significant proliferation response to *** culture of spleen cells showedthat secretion of IFN-γ increased but IL-4 decreased. The results indicate that DNA vaccination bypcDNA3-Sj22.7 is sufficient to elicit significant levels of protective immunity against *** *** DNA vaccine could induce significant cellular and humoral immune response,and display predominantT helper cell type 1 type immune responses,which contribute to the protective immunity against challengeinfection in mice.