Toll-like receptor expression and signaling in human diabetic wounds
Toll-like receptor expression and signaling in human diabetic wounds作者机构：Department of Dermatology University of California at Davis McClellan Outpatient Clinic V A Northern California Health Care System
出 版 物：《World Journal of Diabetes》 (世界糖尿病杂志（英文版）（电子版）)
年 卷 期：2014年第5卷第2期
页 面：219-223页
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：support by ADA Junior Faculty Award to MRD
主 题：Interleukin-1β Inflammation Toll-like receptors 2 Toll-like receptors 4 Tumor necrosis factor-α Type 2-diabetes mellitus Wound healing
摘 要：AIM: To examine the contribution of toll-like receptors(TLRs) expression and activation to the prolonged inflammation often seen in human diabetic ***: Debridement wound tissue was collected from diabetic patients with informed consent. Total RNA and protein were isolated and subjected to real-time polymerase chain reaction and Western blot analyses. RESULTS: TLR1, 2, 4, and 6 mRNA expressions were increased significantly in wounds of diabetic patients compared with non-diabetic wounds(P 0.05). MyD88 protein expression was significantly increased in diabetic wounds compared to non-diabetic wounds. Interleukin-1beta, tumor necrosis factor-alpha concentration nuclear factor-kappa B activation, and thiobarbituric acid reactive substances were increased in diabetic wounds compared to non-diabetic wounds(P 0.01). CONCLUSION: Collectively, our novel findings show that increased TLR expression, signaling, and activation may contribute to the hyper inflammation in the human diabetic wounds.
维普期刊数据库