Evidence for existence of a close association between CD14 and CXCR4 on monocytic cell line U937

作     者：Yang Hengwen Lan Canhui Chen Yinghua~(**) (Laboratory of Immunology,Department of Biology,Tsinghua University Protein Science Laboratory of MOE,Beijing 100084,China) 

作者机构：Laboratory of Immunology Department of Biology Tsinghua University Beijing 100084 China Protein Science Laboratory Beijing 100084 China 

出 版 物：《Progress in Natural Science:Materials International》 (自然科学进展·国际材料(英文))

年 卷 期：2007年第17卷第8期

页      面：979-982页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基　　金：Supported by National Natural Science Foundation of China (Grant Nos.30530680 and 30221003) 

主　　题：CD14 CXCR4 cross-inhibition 

摘      要：The surface expression of HIV-1 coreceptors (CXCR4 and CCR5) on monocytes can be regulated by the ligand of CD14,and the susceptibility of the cells to HIV-1 is then *** previous study found that monoclonal antibody against CD14 could dramatically inhibit CXCR4-mediated chemotaxis and cell-cell *** on these studies,we explored potential relationship between CD14 and CXCR4 on monocytic cell line *** cytometry analysis showed that anti-CXCR4 monoclonal antibody (mAb) 12G5 strongly inhibited binding of the FITC-conjugated anti-CD14 monoclonal antibodies (TUK4 and UCHM1) to U937,while another CX- CR4-specific mAb B-R24 did not show any effect on this *** the other hand,two anti-CD14 monoclonal antibodies (TUK4 and UCH-M1) obviously inhibited the binding of the PE-conjugated anti-CXCR4 mAb 12G5 to U937 but did not inhibit the binding of mAb 12G5 to CXCR4-transfected 3T3 cells (***4),which indicates that the blocking of mAb 12G5 binding to CXCR4 by CD14- specific mAbs is not involved in the possibility that CD14-specific mAbs directly bind to *** results suggested existence of a close association between CD14 and CXCR4 on monocytic cell line U937.