Extraction optimization of coumarins from radix angelicae pubescentis by HPLC-DAD coupled with uniform design

作     者：郭方遒 黄兰芳 周火飞 江秋菊 

作者机构：School of Chemistry and Chemical EngineeringCentral South UniversityChangsha 410083China School of Chemistry and Chemical EngineeringCentral South UniversityChangsha 410083ChinaTechnical Center of Changde Cigarette Factory Changde 415000China 

出 版 物：《Journal of Central South University of Technology》 (中南工业大学学报（英文版）)

年 卷 期：2006年第13卷第2期

页      面：156-159页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 10[医学] 

基　　金：Project(2004714) supported by the Development and Innovation Committee of Hunan Province 

主　　题：radix angelicae pubescentis(Duhuo) couamrins uniform design high performance liquid chromatagraphy-diode array detector(HPLC-DAD) 

摘      要：Uniform design was used to optimize extraction condition of direct refluence extraction of coumarins from the Chinese traditional medicine of radix angelicae pubescentis(Duhuo); the sum peak area of coumarins separated with high performance liquid chromatography(HPLC) at detection wavelength of 320nm was considered as detection index, two factors of solvent concentration and extraction time were mainly studied at extraction temperature of 85℃ and a volume ratio of solvent to sample of 10∶1. Optimal subclass, quadric polynomial step by step aggression and neural network method were applied to process the experimental results. The results show that the first and second methods give the same factors combination (concentration of ethanol: 95%, extraction time: 3.6 h) and the second method is much better than the first one. The extraction model is consequently developed.