Differential Proteomic Analysis of Leaf Development at Rice (Oryza sativa) Seedling Stage

作     者：SHAO Cai-hong LIU Guang-rong WANG Jing-yuan YUE Cai-fei LIN Wen-xiong 

作者机构：Agroecological Institute Fujian Agriculture and Forestry University Fuzhou 350002 P.R.China Soil and Fertilizer Institute Jiangxi Academy of Agricultural Sciences Nanchang 330200 P.R.China 

出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)

年 卷 期：2008年第7卷第9期

页      面：1153-1160页

学科分类：09[农学] 0901[农学-作物学] 

基　　金：the National Natural Science Foundation of China (30600385) the National Key Technology R&D Program of China (2004NZ0104) 

主　　题：rice tiller proteomics the differential expression of proteome 

摘      要：To identify the function of differential expression proteins in different leaves of rice seedlings extracted from 2- to 5-leaf stages, the leaf proteins at the seedling stage of hybrid rice Shanyou 63 were studied by using the approach of plant proteomics, and those proteins were separated with two-dimensional electrophoresis （2-DE） and then analyzed with an imagemaster 2D Elite 5.0. The results showed that the 41 protein spots were detected differential expression, of which 17 new protein spots appeared after the 3-leaf stage, including 9 special protein spots, which were only detected at the 3-leaf stage. Thirteen protein spots increased first and then decreased in expression abundance gradually and finally even disappeared. For the other 11 protein spots, 3 protein spots decreased, but 6 protein spots were opposite in expression abundance, however, 2 protein spots expressed in an irregular pattern after the 2-leaf stage. Of the 41 differential leaf proteins, 15 protein spots were identified by ESI-Q MS/MS and categorized into 4 groups of functions. The results indicated that proteins were the carriers of the functions in cells, but were significantly influenced by the changes in cell function or intercellular environment; hence, the reason that caused the proteomic changes as mentioned earlier might be related to the occurrence of tillers at the rice seedling stage after the 3-leaf stage.