ROCK inhibition enhances neurite outgrowth in neural stem cells by upregulating YAP expression in vitro

作     者：Xu-feng Jia Fei Ye Yan-bo Wang Da-xiong Feng 

作者机构：Department of Orthopedic Surgery Jianyang People's Hospital of Sichuan Province lianyang Sichuan Province China Department of Spine Surgery the Affiliated Hospital of Southwest Medical University Luzhou Sichuan Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2016年第11卷第6期

页      面：983-987页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(General Program) No.30872602 

主　　题：nerve regeneration spinal cord injury neural stem cells Rho signalingpathway neurite outgrowth myelin YAP neural regeneration 

摘      要：Spontaneous axonal regeneration of neurons does not occur after spinal cord injury because of inhibition by myelin and other inhibitory factors. Studies have demonstrated that blocking the Rho/Rho-kinase （ROCK） pathway can promote neurite outgrowth in spinal cord injury models. In the present study, we investigated neurite outgrowth and neuronal differentiation in neural stem cells from the mouse subventricular zone after inhibition of ROCK in vitro. Inhibition of ROCK with Y-27632 increased neurite length, enhanced neuronal differentiation, and upregulated the expression of two major signaling pathway effectors, phospho-Akt and phospho-mitogen-activated protein kinase, and the Hippo pathway effector YAP. These results suggest that inhibition of ROCK mediates neurite outgrowth in neural stem cells by activating the Hippo signaling pathway.