Mechanisms of Apigenin-7-glucoside As a Hepatoprotective Agent

作     者：QIU-SHENGZHENG XI-LINGSUN BoXU GANGLI MENGSONG QIU-SHENG ZHENG≠,*, XI-LING SUN≠, BO XU≠, GANG LI+, AND MENG SONG# ≠Department of biochemistry, Yantai University, Yantai, Shandong 264005, China;Institute of Biophysics, + School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, China;# Hospital of Baiyin City, Baiyin, Gansu 730900, China.

作者机构：DepartmentofbiochemistryYantaiUniversityYantaiShandong264005China InstituteofBiophysicsSchoolofLifeSciencesLanzhouUniversityLanzhouGansu730000China HospitalofBaiyinCityBaiyinGansu730900China. 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2005年第18卷第1期

页      面：65-70页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：This work was supported by the Key Grant Project of the Ministry of Education of China (No.03076) 

主　　题：Apigenin-7-glucoside Malondialdehyde Glutathione 8-Hydroxydeoxyguanosine Hepatotoxicity 

摘      要：Ixeris chinesis (Thunb.) Ankai has been used as a Chinese folk medicine, but only scanty information is available on the physiological and biochemical functions of the compounds extracted from I. chinesis. In the present study the effects of apigenin -7-glucoside (APIG) isolated from I. chinesis against liver injury caused by carbon tetrachloride (CCl4) were investigated. Methods The contents of malondialdehyde (MDA), glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), and reduced glutathione (GSH) were evaluated by spectrophotography. The content of 8-Hydroxydeoxyguanosine (8-OHdG) was measured with high-performance liquid chromatography (HPLC) equipped with electrochemical and UV detection methods. The antioxidant activity of APIG was evaluated using chemiluminescence single photon counting technology. Results CCl4 significantly increased the enzyme activities of GPT and GOT in blood serum, as well as the level of MDA and 8-OHdG in liver tissue, and decreased the levels of GSH. Pretreatment with APIG was able not only to suppress the elevation of GPT, GOT, MDA and 8-OHdG, and inhibit the reduction of GSH in a dose-dependent manner in vivo, but also to reduce the damage of hepatocytes in vitro. On the other hand, we also found that APIG had strong antioxidant activity against reactive oxygen species (ROS) in vitro in a concentration-dependent manner. Conclusion The hepatoprotective activity of APIG is possibly due to its antioxidant properties, acting as scavengers of ROS. These results obtained in vivo and in vitro suggest that APIG has protective effects against hepatic oxidative injury induced by chemicals. Further studies on the pharmaceutical functions and immunological responses of APIG may help its clinical application.