Biological conduits combining bone marrow mesenchymal stem cells and extracellular matrix to treat long-segment sciatic nerve defects

作     者：Yang Wang Zheng-wei Li Min Luo Ya-jun Li Ke-qiang Zhang 

作者机构：Department of OrthopedicsChina-Japan Friendship HospitalJilin University Department of OrthopedicsSecond HospitalJilin University Mathematics SchoolJilin University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2015年第10卷第6期

页      面：965-971页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported by the Science and Technology Development Program of Jilin Province in China No.20110492 

主　　题：nerve regeneration peripheral nerve injury rabbits sciatic nerve injury autologous nerye repair polylactic glycolic acid conduit extracellular matrix gel grafting stress relaxation creep viscoelasticity histomorphology electrophysiology neural regeneration 

摘      要：The transplantation of polylactic glycolic acid conduits combining bone marrow mesenchymal stem cells and extracellular matrix gel for the repair of sciatic nerve injury is effective in some respects, but few data comparing the biomechanical factors related to the sciatic nerve are available. In the present study, rabbit models of 10-mm sciatic nerve defects were prepared. The rabbit models were repaired with autologous nerve, a polylactic glycolic acid conduit ＋ bone marrow mesenchymal stem cells, or a polylactic glycolic acid conduit ＋ bone marrow mesenchymal stem cells ＋ extracellular matrix gel. After 24 weeks, mechanical testing was performed to determine the stress relaxation and creep parameters. Following sciatic nerve injury, the magnitudes of the stress decrease and strain increase at 7,200 seconds were largest in the polylactic glycolic acid conduit ＋ bone marrow mesenchymal stem cells ＋ extracellular matrix gel group, followed by the polylactic glycolic acid conduit ＋ bone marrow mesenchymal stem cells group, and then the autologous nerve group. Hematoxylin-eosin staining demonstrated that compared with the polylactic glycolic acid conduit ＋ bone marrow mesenchymal stem cells group and the autologous nerve group, a more complete sciatic nerve regeneration was found, including good myelination, regularly arranged nerve fibers, and a completely degraded and resorbed conduit, in the polylactic glycolic acid conduit ＋ bone marrow mesenchymal stem cells ＋ extracellular matrix gel group. These results indicate that bridging 10-mm conduit ＋ bone marrow mesenchymal stem sciatic nerve defects with a polylactic glycolic acid cells ＋ extracellular matrix gel construct increases the stress relaxation under a constant strain, reducing anastomotic tension. Large elongations under a constant physiological load can limit the anastomotic opening and shift, which is beneficial for the regeneration and functional reconstruction of sciatic nerve. Better regeneration was fou