OsMADS16 Genetically Interacts with OsMADS3 and OsMADS58 in Specifying Floral Patterning ~in Rice

作     者：Dapeng Yun Wanqi Liang Ludovico Dreni Changsong Yin Zhigang Zhou Martin M. Kater Dabing Zhang 

作者机构：State Key Laboratory of Hybrid Rice School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai 200240 China College of Fisheries and Life Science Shanghai Ocean University Shanghai 201306 China Department of Biosciences Universita degli Studi di Milano Via Celoria 26 20133 Milan Italy 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2013年第6卷第3期

页      面：743-756页

核心收录：

学科分类：0710[理学-生物学] 0821[工学-纺织科学与工程] 090706[农学-园林植物与观赏园艺] 0907[农学-林学] 08[工学] 09[农学] 0901[农学-作物学] 0902[农学-园艺学] 082104[工学-服装设计与工程] 

基　　金：This work was supported by funds from the National Natural Science Foundation of China (31230051 31110103915) National Key Basic Research Developments Program, Ministry of Science and Technology, China (2013CB126902) 863 Hitech Project, Ministry of Science and Technology,China (2011AA10A101 2012AA10A302) the Science and Technology Commission of Shanghai Municipality (10JC1406400 10DZ2294100 11JC1404900) and National Transgenic Major Program (2011ZX08009-003-003). Ludovico Dreni is supported by the BIOGESTECA program financed by the Lombardy region.The authors gratefully acknowledge Drs Hajime Sakai and Yasuo Nagato for providing spwl-1, Dr Venkatesan Sundaresan for providing osmads58, Zhijing Luo and Mingjiao Chen for mutant screening and generation, and Alessandro Merisio for the assistance in in situ analysis. No conflict of interest declared 

主　　题：rice flower B and C genes genetic interaction stamen identity. 

摘      要：Rice （Oryza sativa） has unique floral patterns that contribute to grain yield. However, the molecular mechanism underlying the specification of floral organ identities in rice, particularly the interaction among floral homeotic genes, remains poorly understood. Here, we show that the floral homeotic gene OsMADS16 （also called SUPERWOMAN1, SPWl, a B-class gene） acts together with the rice C-class genes OsMADS3 and OsMADS58 in specifying floral organ patterning. OsMADS16 and the two C-class genes have an overlapping expression pattern in the third whorl founder cells. Compared with the single mutants, both spwl-1 osmads3-4 and spwl-1 osmads58 double mutants exhibit additional whorls of glume-like organs within the flower, particularly an extra whorl of six glume-like structures formed at the position of the wild-type stamens. These ectopic glume-like structures were shown to have palea identity through cellular observation and in situ hybridization analysis using marker genes. Our results suggest that B- and C-class genes play a key role in suppressing indeterminate growth within the floral meristem, particularly whorl-3 primordia. We also hypothesize that, in contrast to previous assumptions, the specialized spikelet organ in rice, the palea, is the counterpart of the sepal in eudicots, and the lemma is homologous to the bract.