Integrated biomarkers in wild crucian carp for early warning of water quality in Hun River, North China

作     者：Binghui Zheng Kun Lei Ruizhi Liu Shuangshuang Song Lihui An 

作者机构：State Key Laboratory of Environmental Criteria and Risk Assessment & State Environmental Protection Key Laboratory of Estuarine and Coastal Research Chinese Research Academy of Environment Sciences 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2014年第26卷第4期

页      面：909-916页

核心收录：

学科分类：07[理学] 08[工学] 09[农学] 0815[工学-水利工程] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：supported by the Water Pollution Control and Management(No.2009ZX07528) 

主　　题：real time quantitative reverse  transcription polymerase chain reaction Carassius auratus Hun River integrated biomarker response water quality 

摘      要：Metabolizing enzymes play important roles in the detoxification of various pollutants in aquatic organisms, thereby they can also be used to provide early-warning signals of environmental risks. Real-time quantitative reverse-transcription polymerase chain reaction assays were developed to quantify cytochrome P450 1A （CYP1A）, superoxide dismutase （SOD）, glutathione peroxidase （GPx）, catalase （CAT）, and glutathione-S-transferase （GST） in crucian carp （Carassius auratus）. The methods were then used to detect the respective mRNA expression levels in liver tissue in wild crucian carp from the Hun River, North China. CYP1A mRNA expression was significantly up-regulated in fish from stations $5, $6, and $8 （p 〈 0.05）. SOD mRNA expression was significantly down-regulated in downstream areas relative to fish from upstream sites （p 〈 0.05）; GPx and CAT mRNA expression levels were also down-regulated at $9 （p 〈 0.05）. In contrast, GST mRNA expression showed no obvious change between fish collected from up- or downstream areas of the river. Finally, an integrated biomarker response was used to evaluate the integrated impact of pollutants in the Hun River and allow better comprehension of the real toxicological risk of these investigated sites.