Aberrant expression and function of TCF4 in the proliferation of hepatocellular carcinoma cell line BEL-7402

作     者：Dong Hong ZHAO, Jian Jun HONG, Shi Ying GUO, Run Lin YANG, Jun YUAN, Chuan Jun WEN, Kai Ya ZHOU, Chao Jun LI~* The Jiangsu Key Laboratory of Molecular Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210097, Jiangsu, China. Dong Hong ZHAO, Jian Jun HONG, Shi Ying GUO, Run Lin YANG, Jun YUAN, Chuan Jun WEN, Kai Ya ZHOU, Chao Jun LI~* The Jiangsu Key Laboratory of Molecular Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210097, Jiangsu, China.

作者机构：Li CJ Nanjing Normal Univ Coll Life Sci Jiangsu Key Lab Mol Med Biotechnol Nanjing 210097 Peoples R China 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：2004年第14卷第1期

页      面：74-80页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：国家自然科学基金(2000SWX000B501) 

主　　题：Wnt signaling pathway β-catenin dominant negative TCF4. 

摘      要：Wnt signaling pathway is essential for development and tumorigenesis, however, this signaling pathway in the progress of hepatocellular carcinoma (HCC) remains unclear. In this paper, we studied the function of human T-cell transcription factor-4 (TCF4), a key factor of Wnt signaling pathway, on the proliferation of HCC cell line. We showed that the expression of TCF4 mRNA in HCC cell line BEL-7402 was higher than that in immortalized normal liver cell line L02. Blockage of Wnt pathway by △NTCF4, a dominant negative TCF4, could suppress BEL-7402 cells growth and decrease the expression of cyclin D1 and c-myc, two of target genes of Wnt pathway. On the other hand, stimulating Wnt pathway by introducing a degradation-resistant β-catenin S37A could increase BEL-7402 cells proliferation. But all the treatments had no effect on L02 cells. Our data indicated that TCF4 might be another key factor in Wnt pathway involved in HCC cells proliferation and TCF4 could be an effective therapeutic target for suppress