Cloning of human brevican cDNA and expression of its mRNA in human glioma

作     者：韩晞 董艳 由振东 何成 卢亦成 

作者机构：Department of Neurosurgery Changzheng Hospital Second Military Medical University Shanghai 200003 China Department of Neurobiology College of Basic Medical Sciences Second Military Medical University Shanghai 200433 China 

出 版 物：《Journal of Medical Colleges of PLA(China)》 (中国人民解放军军医大学学报（英文版）)

年 卷 期：2003年第18卷第6期

页      面：388-392页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：brevican RT-PCR in situ hybridization glioma 

摘      要：Objective: To clone the cDNA of human brevican secreting isoform and to investigate its mRNA expression in human glioma. Methods: The full-length cDNA of human brevican secreted isoform was cloned from a human anaplastic astrocytoma by RT-PCR, and the expression of human brevican mRNA in 22 cases of human glioma and 13 cases of non-glial brain tumors were investigated by in situ hybridization. Results: The cDNA which including the whole open reading frame of human brevican secreted isoform was obtained. In situ hybridization showed that brevican positive cells were present in all of the 22 cases of gliomas (100%), whereas none were found in the 13 cases of non-glial and metastasis brain tumors examined. Conclusion: The results suggest that brevican mRNA is highly and specifically expressed in human glioma.