Screening Psychrophilic Fungi of Cellulose Degradation and Characteristic of Enzyme Production

作     者：Wang Da-qing Jin Wen-ran Sun Tai-peng Meng Yu-tian Zhao Wei Wang Hong-yan 

作者机构：Heilongjiang Economic Research Institute of State Farms Harbin 150030 China College of Resources and Environment Northeast Agricultural University Harbin 150030 China 

出 版 物：《Journal of Northeast Agricultural University(English Edition)》 (东北农业大学学报（英文版）)

年 卷 期：2016年第23卷第2期

页      面：20-27页

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

基　　金：Supported by the Post-doctoral Research Starting Fund of Heilongjiang Province(LBH-Q13020) 

主　　题：low temperature cellulose degrading fungi screening enzyme activity 

摘      要：A fungus（WR-C1） decomposed cellulose was isolated from a hypothermal litter layer using Congo red medium as the preliminary screening culture medium and then using a filter as the secondary screening medium at low temperature. The experiment showed that the weight loss rate of filter paper on the 15 th days could reach 30.69%. A morphologic and ITS gene sequence analysis suggested that CF-C1 was Cladosporium. We mainly studied the effects of culture time, inoculation amount, initial p H and different sources of carbon, nitrogen and inorganic salt on the cellulase production of strain WR-C1. Under optimum cultural condition, the highest value of WR-C1 enzyme production and filter paper enzyme were 3.27 U · m L～（-1） and 0.51 U · m L～（-1）.