Isolation, purification and structural characterization of a water-soluble polysaccharide HM41 from Halenia elliptica ***
Isolation, purification and structural characterization of a water-soluble polysaccharide HM_(41) from Halenia elliptica ***作者机构:College of Life and Environmental ScienceMinzu University of ChinaBeijing 100081China Beijing Engineering Research Center of Food Environment and Public HealthMinzu University of ChinaBeijing 100081China Institute of ChemistryChinese Academy of SciencesBeijing 100080China
出 版 物:《Chinese Chemical Letters》 (中国化学快报(英文版))
年 卷 期:2016年第27卷第6期
页 面:979-983页
核心收录:
学科分类:1006[医学-中西医结合] 1005[医学-中医学] 100513[医学-民族医学(含:藏医学、蒙医学等)] 100602[医学-中西医结合临床] 10[医学]
基 金:supported by the First-Class Discipline Construction Project for First-Class University of Minzu University of China (No. YLDX01013),111 Project (No. B08044) The ecological conservation technology integration and demonstration in north of Dzungaria (No. 2014BAC15B04)
主 题:Halenia elliptica D.Don Polysaccharide Isolation purification Structural analysis Antioxidant activities
摘 要:A water-soluble polysaccharide, HM(41), was obtained from Halenia elliptica D. Don by acidic ethanol fractionation and gel filtration. Its homogeneity was confirmed by chromatography using multiple systems. HM(41) was composed of rhamnose(Rha), arabinose(Ara), xylose(Xyl), mannose(Man),galactose(Gal), glucose(Glc) with a molar ratio of 1.0:5.5:1.8:3.0:9.4:21. The average molecular weight of HM(41) was approximately 1.17 * 10~4. Periodate oxidation, Smith degradation, methylation and GC, IR,NMR, XRD, GC–MS analysis were used for the structural analysis of HM(41). Its main chain was composed mainly of β-(1→4)Gal, β-(1→4)Glc and β-(1→6)Glc. β-(1 →4)Gal were substituted at 6-O and on average there were 14 branches among 23 main chain residues;(1→ 4)Glc had no branch;(1→6)Glc were substituted at 3-O and on average there were 9 branches among 14 main chain residues. The side chain was composed of(1→3,6)-Rha,(1→4)/(1→5)-Ara,(1 →4)/(1→5)-Xyl,(1→4,6)-Man and(1→2)-Glc. The terminal residue was composed of Ara, Xyl, Man, Gal, and Glc. Then, we demonstrated that HM and HM(41) had strong scavenging activities in vitro hydroxyl. Overall, HM and HM(41) may have potential applications in the antioxidants for medical and food industry.