Genomic insights into the ESBL and MCR-l-producing ST648 Escherichia coli with multi-drug resistance

作     者：Huimin Zhang Christopher H. Seward Zuowei Wu Huiyan Ye Youjun Feng 

作者机构：Department of Medical Microbiology and Parasitology Zhejiang University School of Medicine Hangzhou 310058 China Carl R. Woese Institute for Genomic Biology University of Illinois at Urbana-Champaign Urbana IL 61801 USA Department of Veterinary Microbiology and Preventive Medicine Iowa State University Ames IA 50010 USA 

出 版 物：《Science Bulletin》 (科学通报（英文版）)

年 卷 期：2016年第61卷第11期

页      面：875-878页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1007[医学-药学(可授医学、理学学位)] 090601[农学-基础兽医学] 100706[医学-药理学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 09[农学] 0906[农学-兽医学] 071007[理学-遗传学] 0836[工学-生物工程] 

基　　金：This work was supported by Zhejiang Provincial Natural Science Foundation for Distinguished Young Scholars (LR15H190001)  the National Natural Science Foundation of China (31570027)  and a start-up package from Zhejiang University (Y.F.). Dr. Feng is a recipient of the "Young 1000 Talents" Award 

主　　题：MCR-1 Extended-spectrum beta-lactam(ESBL) Colistin resistance ST648 

摘      要：Polymyxin acts as an ultimate line of refuge against the severe infections by multidrug-resistant Gram- negative pathogens. This conventional idea is challenged dramatically by the recent discovery of mobile colistin resistance gene （mcr-1） is prevalent in food animals and human beings worldwide. More importantly, the mcr-1 gene was found to be co-localized with other antibiotic resistance genes, raising the possibility that super-bugs with pan-drug resistance are emerging. However, little is reported on the genomes of the mcr-l-positive bacterial host reservoirs. Here we report genome sequencing of three human isolates of the mcr-l-positive Escherichia coli （E15004, E15015 and E15017） and define general features through analyses of bacterial comparative genomics. Fur- ther genomic mining together with sequence typing allowed us to elucidate that the MCR-l-carrying E. coli E15017 belongs to the sequence type ST648 and copro- duces extended-spectrum β-1actamase （ESBL）. Given the fact that ST648 has been known to associate New Delhi metallo-β-1actamase 1 or ESBL, with either our results highlighted the possibility of ST648 as an epidemic clone with multidrug resistances.