Correlation of estrogen receptor alpha gene polymorphisms and bone mineral density in Chinese women with chronic periodontitis

作     者：ZHANG Xuan DAI Juan LONG Yin WU Hao LI Xiao-juan DING Yin 

作者机构：Department of Orthodontics School of Stomatology Fourth Military Medical University Xi'an Shaanxi 710032 China Department of Traditional Chinese Medicine Fourth Military Medical University Xi'an Shaanxi 710032 China Laboratory of Bone Density Fourth Military Medical University Xi'an Shaanxi 710032 China Xijing Hospital Statistical Analysis Laboratory Fourth Military Medical University Xi'an Shaanxi 710032 China Department of Stomatology Chinese People's Liberation Army General Hospital Beijing 100853 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2010年第123卷第22期

页      面：3262-3267页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：This study was supported in part by the a grant from National Natural Science Foundation of China (No. 30572069).Acknowledgements: The authors are grateful to Prof. LIU Jia-yun  Department of Clinical Molecular Biology  Xijing Hospital  Fourth Military Medical University  for his technical advice 

主　　题：estrogen receptor-a gene polymorphisms chronic periodontitis bone mineral density pre-menopausal post-menopausal osteoporosis 

摘      要：Background Periodontitis and osteoporosis are one of the frequently encountered diseases in post-menopausal women. Estrogen receptors （ERs） regulated bone metabolism. To investigate the possible effect of ER-elpha （a） gene polymorphisms on bone mineral density （BMD） in pre- and post-menopausal Chinese women with chronic periodontitis （CP）, we provided sufficient quantitative information concerning the correlation between ER gene polymorphisms and BMD in periodontitis. Methods Sixty-five post-menopausal and eighty pre-menopausal CP women, and sixty post-menopausal healthy individuals were recruited in this study. Genomic DNA was extracted from oral mucosa swab sample of each subject by the Chelex-100 method. Determination of the ER-a polymorphisms was performed by polymerese chain reaction-restriction fragment length polymorphism （PCR-RFLP） technique with Xbal and Pvull enzyme. The index for periodontal examination includes clinical attachment loss （CAL） and probing pocket depth （PPD）. BMD was measured by dual-energy X-ray absorptiometry （DEXA）. Results There were no significant differences between the ER-α genotypes of Pvull and Xbal and BMD in post-menopausal and pre-menopausal CP patients, respectively （P 〉0.05）. However, there was association between pre- and post-menopausal CP patients at BMD of lumbar spine L2-L4 （P=0.027） and Ward＇s BMD （P=0.004）. Furthermore, the post-menopausal CP women who carried Pvull TT genotype presented significantly lower Ward＇s BMD than the pre-menopausal CP women （P=-0.007）, meanwhile, the post-menopausal CP women who carried Xbal AA genotype presented significantly lower spine L2-L4 BMD than the pre-menopausal CP women （P=0.003）. Conclusions ER-α gene polymorphisms may be a susceptible indicator for BMD variation of lumbar spine L2-L4 and Ward in Chinese pre- and post-menopausal women patients with CP.