Mechanism involved in Danshen-induced fluid secretion in salivary glands

作     者：Fei Wei Mu-Xin Wei Masataka Murakami 

作者机构：Division of Physiological SciencesSchool of Life ScienceGraduate University for Advanced StudiesOkazaki 444-8585Japan Department of Traditional Chinese MedicineThe first Affiliated Hospital with Nanjing Medical University Division of Cellular PhysiologyNational Institute for Physiological SciencesNational Institutes of Natural SciencesOkazaki 444-8787Japan 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2015年第21卷第5期

页      面：1444-1456页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by Grants from The National Institute for Physiological Sciences and Graduate University for Advanced Studies 

主　　题：Salivary fluid secretion Xerostomia Chinese herb D 

摘      要：AIM:Danshen s capability to induce salivary fluid secretion and its mechanisms were studied to determine if it could improve ***:Submandibular glands were isolated from male Wistar rats under systemic anesthesia with pentobarbital *** artery was cannulated and vascularly perfused at a constant *** excretory duct was also cannulated and the secreted saliva was weighed in a cup on an electronic *** weight of the accumulated saliva was measured every 3 s and the salivary flow rate was *** addition,the arterio-venous difference in the partial oxygen pressure was measured as an indicator of oxygen *** order to assess the mechanism involved in Dansheninduced fluid secretion,either ouabain(an inhibitor of Na+/K+ ATPase) or bumetanide(an inhibitor of NKCC1) was additionally applied during the Danshen *** order to examine the involvement of the main membrane receptors,atropine was added to block the M3 muscarinic receptors,or phentolamine was added to block the α1 adrenergic *** order to examine the requirement for extracellular Ca2+,Danshen was applied during the perfusion with nominal Ca2+ free ***:Although Danshen induced salivary fluid secretion,88.7 ± 12.8 μL/g-min,n = 9,(the highest value around 20 min from start of DS perfusion was significantly high vs 32.5 ± 5.3 μL/g-min by carbamylcholine,P = 0.00093 by t-test) in the submandibular glands,the time course of that secretion differed from that induced by *** was a latency associated with the fluid secretion induced by Danshen,followed by a gradual increasein the secretion to its highest value,which was in turn followed by a slow decline to a near zero *** application of either ouabain or bumetanide inhibited the fluid secretion by 85% or 93%,and suppressed the oxygen consumption by 49% or 66%,*** results indicated that Danshen activates Na+/K+ ATPase and NKCC1 to maintain Cl- release and K+ r