Characterization of marine microplankton communities of Qingdao coastal areas using randomly amplified polymorphic DNA(RAPD)

作     者：GAO Shan HU Xiaozhong CHEN Zigui XU Henglong YI Zhenzhen LIN Xiaofeng LI Jiqiu GAO Shan1,HU Xiaozhong1,CHEN Zigui1,XU Henglong1,YI Zhenzhen1,LIN Xiaofeng2,LI Jiqiu21Laboratory of Protozoology,Key Laboratory of Mariculture,Ocean University of China,Qingdao 266003,China2Laboratory of Protozoology,College of Life Science,South China Normal University,Guangzhou 510631,China

作者机构：Laboratory of Protozoology Key Laboratory of Maricuhure Ocean University of China Qingdao 266003 China Laboratory of Protozoology College of Life Science South China Normal University Guangzhou 510631 China 

出 版 物：《Acta Oceanologica Sinica》 (海洋学报（英文版）)

年 卷 期：2009年第28卷第1期

页      面：55-61页

核心收录：

学科分类：0710[理学-生物学] 0908[农学-水产] 07[理学] 0707[理学-海洋科学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 071002[理学-动物学] 090102[农学-作物遗传育种] 

基　　金：The National Natural Science Foundation of China under contract Nos 30570236,U0633006,40506033 and 40676076 the Program for New Century Excellent Talents in Universities of China under contract No. NCET05-0595 

主　　题：marine plankton RAPD community diversity phylogeny 

摘      要：Microplankton communities of three coastal sites of Qingdao,Shandong Province,China were investigated using RAPD（random amplified polymorphic DNA） molecular markers and morphological *** RAPD-primers were selected to amplify the DNA *** genetic distances inferred from the pairwise similarities were calculated for the phylogenetic tree ***,the traditional microscopic determination,a way of visualizing the species composition,was performed to detect the major taxa of microplanktons from all *** showed that：（1） the band sharing index values were in the range of 0.504 2-0.763 2 among samples from the same sampling site at different time scales,while 0.406 5-0.685 7 among the samples from different stations at the same time scales,indicating that spatial variations of microplankton communities were more pronounced than temporal ones;（2） samples from the same station basically clustered together,corresponding to the geographic distribution of the sampling sites;（3） diversity derived from genetic and morphological data did not correspond with each other well.