Stimulation of mucin secretion from human bronchial epithelial cells by mast cell chymase

作     者：Shao-heng HE1,2,3, Jian ZHENG1 1Allergyand Inflammation Research Institute, Shantou University MedicalCollege, Shantou 515031, China 2ImmunopharmacologyGroup, Southampton General Hospital, Southampton, UK 

作者机构：Allergy and Inflammation Research Institute Shantou University Medical College Shantou 515031 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2004年第25卷第6期

页      面：125-130页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：We are grateful for the financial support from Li Ka Shing Foundation  Hong Kong  China  No C0200001 

主　　题：chymase mucins mast cells epithelial cells MUC5AC protein 

摘      要：AIM: To investigate the effect of chymase on the mucin secretion from human bronchialepithelial cells. METHODS: Primarily-cultured human bronchial epithelial (PCHBE) cells and normal human bronchial epithelial (NHBE) cells were cultured with chymase or other stimulus in a mixture of bronchial epithelial growth medium (BEGM) and Dulbecco’s modified Eagle’s medium (DMEM), and the quantities of stimulatory mucin release were recorded. MUC5AC mucin was measured with an ELISA and dolichos biflorus agglutinin (DBA) mucin was determined with an enzyme linked DBA assay. RESULTS: A dose-dependent secretion of DBA mucin from PCHBE cells was observed with chymase with a maximum secretion of 98 % above baseline being achieved following 3 h incubation. The action of chymase started from 1 h, peaked at 3 h and dramatically decreased at 20 h following incubation. Chymase was able to also stimulate approximately 38 % increase in MUC5AC mucin release from PCHBE cells, and about 121 % increase in DBA mucin release from NHBE cells. A chymase inhibitor soybean trypsin inhibitor (SBTI) was able to inhibit up to 85 % chymase induced mucin release, indicating that the enzymatic activity was essential for the actions of chymase on bronchial epithelial cells. CONCLUSION: Chymase is a potent stimulus of mucin secretion from human bronchial epithelial cells. It can contribute to mucus hypersecretion process in the patients with chronic obstructive pulmonary disease or asthma.