Ploidy-, gender-, and dose-dependent alteration of selected biomarkers in Clarias gariepinus treated with benzo[a]pyrene

作     者：Ali Karami Swee J.Teh Mohamad Pauzi Zakaria Simon C.Courtenay 

作者机构：Laboratory of Aquatic ToxicologyFaculty of Medicine and Health SciencesUniversiti Putra Malaysia School of Veterinary MedicineDepartment of AnatomyPhysiologyand Cell BiologyUniversity of California Centre of Excellence for Environmental ForensicsFaculty of Environmental StudiesUniversiti Putra Malaysia Department of Environment and Resource StudiesCanadian Water NetworkCanadian Rivers InstituteUniversity of Waterloo 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2015年第27卷第12期

页      面：95-102页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 0713[理学-生态学] 

主　　题：Triploid Diploid Male Female Fish biomarkers 

摘      要：Naturally-occurring and artificially-induced polyploids have been documented in various fish species but to date no comparison has been reported of the impacts of ploidy on fish biomarker responses to organic pollutants.This study describes effects of ploidy,gender,and dose on biliary fluorescent aromatic compound（FAC） concentrations,hepatic ethoxyresorufin-O-deethylase（EROD） and glutathione S-transferase（GST） activities in one of the most commonly cultured warm-water species,the African catfish Clarias gariepinus.Recently matured male and female diploid and triploid fish were intraperitoneally（i.p.）injected with 0,5 or 25 mg/kg benzo[a]pyrene（BaP） and liver and gallbladder were sampled48 hr later.No significant differences were found between ploidies in bile concentrations of7,8 dihydrodiolbenzo[a]pyrene（7,8D BaP）,1-hydroxybenzo[a]pyrene（1-OH BaP） or3-hydroxybenzo[a]pyrene（3-OH BaP）.However,concentrations of the biliary FACs did differ between males and females at different dose of injection with generally higher concentrations in females at the low dose of BaP and higher concentrations in males at the higher BaP concentration.Hepatic EROD activity did not exhibit gender-dependent difference,whereas it was significantly higher in triploids than diploids.GST activities were not significantly influenced by any of the tested factors.This work advanced our understanding of the role of ploidy,gender,and dose in biotransformation of pollutants in fish.