Effects of aspirin on the expression of nuclear factor-κB in a rat model of acute pulmonary embolism

作     者：Ling-cong Wang Rong-lin Jiang Wei Zhang Li-ling Wei Ru-hui Yang 

作者机构：Intensive Care UnitFirst Aff iliated Hospital of Zhejiang University of Traditional Chinese Medicine Zhejiang University of Traditional Chinese Medicine Hangzhou Hebei Science & Technology Co.Ltd 

出 版 物：《World Journal of Emergency Medicine》 (世界急诊医学杂志（英文）)

年 卷 期：2014年第5卷第3期

页      面：224-228页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by grants from the Natural Science Foundation of Zhejiang Province(Y207052,LY12H29005) the Construction of Key Disciplines in Traditional Chinese Medicine of Zhejiang Province(2012-XK-A12) 

主　　题：Aspirin Acute pulmonary embolism Nuclear factor-κB 

摘      要：BACKGROUND:Acute pulmonary embolism(APE) is a disorder involving the pulmonary circulation resulting from a blockage of the pulmonary artery. The present study aimed to investigate the effects of aspirin on the nuclear factor-κB(NF-κB) activity in a rat model of ***:A total of 108 healthy male Sprague-Dawley rats were randomly assigned into six groups(n=18 rats per group):control group,sham operation group,APE model group,and low-,medium- and high-dose aspirin groups. Six,24,and 72 hours after the induction of APE,rats in the low-,medium- and high-dose aspirin groups were given aspirin at a respective daily dose of 150,300,and 600 mg/kg by gavage for three consecutive days. Rats in the other groups were treated with equal volumes of normal saline. Six rats in each group were anesthetized with 10% chloral hydrate solution at each time point,and then the lung tissues were collected and analyzed using immunohistochemical ***:Positive immunohistochemical staining was present in the bronchial epithelial cells,alveolar cells,macrophages,and surrounding bronchial smooth muscle cells. When compared with the APE model group,the number of positive cells was signif icantly lower in the other groups at each time point(P0.001). Statistically signif icant differences were also observed among the aspirin-treated groups at 6 hours(P0.05,P0.001). Compared with the APE model group,NF-κB protein expression was reduced in the other groups at each time point(P0.05,P0.001). Rats from the APE model group had thrombosis,damaged alveolar walls,and pulmonary hemorrhage,along with different degrees of inf lammatory cellular inf iltration at each time point. However,pathological changes such as pulmonary hemorrhage and inf iltration of inf lammatory cells were attenuated after the aspirin ***:Aspirin can signifi cantly inhibit NF-κB activity in the lung of rats with APE in a dose-dependent manner,and can alleviate lung injury after APE.