INFLUENCE OF MIXING DEVICE ON SERUM-FREE CULTIVATION OF INSECT CELLS IN SPINNER FLASKS

作     者：Zhao Jiao, Dai Hu and Tan Wensong (State Key Lab of Bioreactor Engineering, East China University of Science and Technology Shanghai 200237,China) 

出 版 物：《化工学报》 (CIESC Journal)

年 卷 期：2000年第51卷第S1期

页      面：101-104页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 

主　　题：serum-free insect cells adaptation spinner flasks culture environment 

摘      要：A culture system was developed and successfully employed for the serum-free cultivation of Tn-5B1-4 (Tn5 ) insect cells. With our adaptation procedure, it was possible to obtain cells fully adapted to serum-free media in stationary T-flasks and then enable these adapted cells to grow well in spinner flasks immediately. The spinner ask with special stirring design proved tO provide favorable culture environment that made it desirable for use in the serum-free cultivation of Tn5 cells even at low seeding density.