Nucleic acid crystallization and X-ray crystallography facilitated by single selenium atom

作     者：Wen Zhang Jack W. Szostak Zhen Huang 

作者机构：Department of Chemistry Georgia State University Atlanta GA 30303 USA Howard Hughes Medical Institute Department of Molecular Biology and Center for Computational and Integrative Biology Massachusetts General Hospital Boston MA 02114 USA College of Life Sciences Sichuan University Chengdu 610041 China 

出 版 物：《Frontiers of Chemical Science and Engineering》 (化学科学与工程前沿（英文版）)

年 卷 期：2016年第10卷第2期

页      面：196-202页

核心收录：

学科分类：090403[农学-农药学(可授农学、理学学位）] 070207[理学-光学] 07[理学] 08[工学] 09[农学] 0904[农学-植物保护] 0803[工学-光学工程] 0702[理学-物理学] 

主　　题：selenium DNA RNA nucleic acid crystallization 

摘      要：X-ray crystallography is a powerful strategy for 3-D structure determination of macromolecules, such as nucleic acids and protein-nucleic acid complexes. However, the crystallization and phase determination are the major bottle-neck problems in crystallography. Recently we have successfully developed synthesis and strategy of selenium-derivatized nucleic acids （SeNA） for nucleic acid crystallography. SeNA might not only provide the rational strategies to solve the phase determination problem, but also offer a potential strategy to explore crystallization solutions.