Phenotypic Characterization of Indigenous Iraqi Sinorhizobium meliloti Isolates for Abiotic Stress Performance
Phenotypic Characterization of Indigenous Iraqi Sinorhizobium meliloti Isolates for Abiotic Stress Performance作者机构：Biology Department AI-Mustanserya University Baghdad Iraq Biology Department Baghdad University Baghdad Iraq Plant Biotechnology Department Biotechnology Research Center Al-Nahrain University Baghdad Iraq
出 版 物：《Journal of Life Sciences》 (生命科学（英文版）)
年 卷 期：2014年第8卷第1期
页 面：1-9页
学科分类：0710[理学-生物学] 090603[农学-临床兽医学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 09[农学] 0906[农学-兽医学] 071005[理学-微生物学] 10[医学]
主 题：Sinorhizobium meliloti phenotypic abiotic stresses.
摘 要：Alfalfa （Medicago sativa L.） is being grown in harsh environment in Iraq and is mostly subjected to abiotic stresses such as drought, salinity, pH and temperature. Both alfalfa and its nitrogen fixing symbiotic bacteria Sinorhizobium meliloti are affected by these environmental stresses. Enhancing nitrogen fixation biologically could be achieved through selection of tolerant strains of S. meliloti to these environmental stresses and inoculating them to the crop, also growing tolerant cultivars. This study examines phenotypic diversity for tolerance to drought, salinity, temperature and pH. Sixty isolates sampled from different areas of Iraq. The results revealed high degree of phenotypic diversity in Sinorhizobium populations. Furthermore, the isolates which showed tolerance to drought stress also showed tolerance to salinity and high degree of temperature, indicating direct relationship between three physiological path ways. Also 58.3% of drought tolerant isolates were alkaline tolerant they tolerated up to pH 9, point to say almost all drought tolerant isolates in this study illustrated strong ＋ positive reaction to catalase enzyme. And 91.6% themes were negative for Gelatinase enzyme test. While only 50% of drought sensitive isolates were negative for drought sensitive isolates could grow at high temperature （42 ℃）.
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