Subcloning and Expression of Human Papillomavirus Type 16 E2 Gene in ***

作     者：何玉凯 徐钤 He Yukai Xu Qian Department of Biochemistry,First Military Medical University,Guangzhou

作者机构：Department of BiochemistryFirst Military Medical UnioersityGuangzhou 

出 版 物：《Journal of Medical Colleges of PLA(China)》 (中国人民解放军军医大学学报（英文版）)

年 卷 期：1989年第4卷第2期

页      面：163-168页

学科分类：10[医学] 

主　　题：human papillomavirus transactivation cell transformation cloning and expression rocombinant DNA 

摘      要：By using molccular doning technique,the E2 gene of human papillomavirus type 16 wasexpressed in *** 3.2 kb fragment containing the E2 gene was cut out from HPV16 genomeand blunted with nuelease *** plasmid pBD2 DNA was linearized with Hind Ⅲ and bluntedwith nuclasc S1 *** ligation,thc ligsted DNA was used to transform *** BMH *** positive colonies were screened by in situ hybridization technique,and proceedcd to DNA analy-sis and proton *** purified expressed protein was used to run immunoclctrophoreesis withantiserum against *** concktudcd that thc expressed protcin was a fusion protein ofbeta-galae-sidasc-E2 protein.