Enteroaggregative Escherichia aoliisolated from Chinese diarrhea patients with high-pathogenicity island of Yersinia'xs involved in synthesis of siderophore yersiniabactin
Enteroaggregative Escherichia aoliisolated from Chinese diarrhea patients with high-pathogenicity island of Yersinia'xs involved in synthesis of siderophore yersiniabactin作者机构：Department of Epidemiology Southern Medical College Guangzhou 510515 Guangdong Province China Department of Genetics Institute of Epidemiology and MicrobiologyChinese Center for Disease Control and Prevention Beijing 102206 China Department of Infectious Diseases Nanfang Hospital Southem Medical College Guangzhou 510515 Guangdong Province China
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2005年第11卷第37期
页 面：5816-5820页
核心收录：
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100401[医学-流行病与卫生统计学] 10[医学]
基 金：Supported by the National Basic Research Program of Ministry of Science and Technology of China No. G1999054101
主 题：High-pathogenicity island Enteroaggregative Escherichia colt, Yersiniabactin
摘 要：AIM: To investigate the distribution of 12 high-pathogenicity island (HPI) genes and the relation between HPI genes and expression of yersiniabactin (Ybt) in enteroaggregative ***(EAggEC) isolated from Chinese diarrhea ***: The distribution of 12 HPI genes was investigated by PCR and DNA hybridization in two prototype strains of EAggEC, EAggEC 17-2, EAggEC O42, and 6 clinical EAggEC isolates from China. The production of siderophore Ybt in HPI-positive strains was detected by reporter gene bioassay to determine the relation between HPI genes and expression of Ybt. Flow cytometry was used to detect fluorescent signal of the reporter strain that could designate production of ***: Seven strains were HPI-positive and one strain was HPI-negative. Six of the seven HPI-positive strains were inserted into asnT-tRNA site. Moreover, seven EAggEC HPI-positive strains revealed enhanced fluorescence signal but the EAggEC HPI-negative strain did not. However, there was a difference in Ybt expression condition and level among these seven EAggEC HPI-positive strains. Although UFT073 strain, the prototype strain of uropathogenic ***(UPEC), carried the complete HPI core part, we did not detect the expression of Ybt in ***: EAggEC HPI-positive strains can express the Ybt system, but the presence of HPI core part does not mean the functional expression of Ybt.
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