Shenmai Injection Reduces Cardiomyocyte Apoptosis Induced by Doxorubicin through miR-30a/Bcl-2
作者机构：Department of Cardiovascular Medicine National Clinical Research Center for Chinese Medicine Cardiology Xiyuan Hospital China Academy of Chinese Medical Sciences Department of Integrated Traditional Chinese and Western Medicine Tianjin Medical University Cancer Institute and Hospital Department of Cardiovascular Medicine First Teaching Hospital of Tianjin University of Traditional Chinese Medicine Department of Cardiovascular Medicine Tianjin Key Laboratory of Traditional Research of Traditional Chinese Medicine Prescription and Syndrome
出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志)
年 卷 期：2025年第3期
页 面：240-250页
核心收录：
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：Supported by the National Natural Science Foundation of China (No. 81904118) Hospital Capability Enhancement Project of Xiyuan Hospital,China Academy of Chinese Medical Sciences (No. XYZX0201-09) the Special Programme for the Cultivation of Outstanding Young Scientific and Technological Talents of the China Academy of Chinese Medical Sciences (No. ZZ17-YQ-005)
摘 要：Objective: To explore the molecular mechanism of Shenmai Injection(SMI) against doxorubicin(DOX) induced cardiomyocyte apoptosis. Methods: A total of 40 specific pathogen-free(SPF) male Sprague Dawley(SD) male rats were divided into 5 groups based on the random number table, including the control group,the model group, miR-30a agomir group, SMI low-dose(SMI-L) group, and SMI high-dose(SMI-H) group, with 8 rats in each group. Except for the control group, the rats were injected weekly with DOX(2 mg/kg) in the tail vein for 4 weeks to induce myocardial injury, and were given different regimens of continuous intervention for 2 *** function was detected by echocardiography and myocardial pathological changes were observed by Van Gieson(VG) staining. Myocardial injury serum markers, including creatine kinase(CK), lactate dehydrogenase(LDH), troponin T(c Tn T), N-terminal pro-brain natriuretic peptide(NT-pro BNP), soluble ST2(sST2), and growth differentiation factor-15(GDF-15) were detected by enzyme linked immunosorbent assay(ELISA). Cardiomyocyte apoptosis was observed by terminal deoxynucleotidyl transferase-mediated biotinylated d UTP triphosphate nick end labeling(TUNEL) and transmission electron microscopy, and the expressions of target proteins and m RNA were detected by Western blot and quantitative real time polymerase chain reaction(qRT-RCR), ***: The treatment with different doses of SMI reduced rat heart mass index and left ventricular mass index(P0.05), significantly improved the left ventricular ejection fraction(P0.05), decreased the levels of serum CK,LDH, cTnT, and NT-proBNP(P0.05 or P0.01), reduced the levels of serum sST2 and GDF-15(P0.05 or P0.01), decreased the collagen volume fraction, reduced the expressions of rat myocardial type Ⅰ and type Ⅲcollagen(P0.05 or P0.01), and effectively alleviated myocardial fibrosis. And the study found that SMI promoted the expression levels of miR-30a and Bcl-2 in myocardium, and d
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