DNMT1 promotes the proliferation and migration of gastric cancer cells by inducing microRNA-125a-5p methylation to promote SERPINE1 protein

作     者：Hui Xie Hui Wang Ru-Hong Li Yue-Wen Zhang Xi-Rui Fan Xiao-Xue He Ao-Ran Guan 

作者机构：Department of General SurgeryYan’an Hospital of Kunming CityKunming 650051Yunnan ProvinceChina Department of Digestive Internal MedicineYan’an Hospital of Kunming CityKunming 650051Yunnan ProvinceChina 

出 版 物：《World Journal of Gastrointestinal Oncology》 (世界胃肠肿瘤学杂志(英文))

年 卷 期：2025年第17卷第3期

页      面：188-201页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the Research Program of the Science and Technology Department of Yunnan Province No.202101AY070001-204 

主　　题：Gastric cancer microRNA-125a-5p DNA methyltransferase 1 SERPINE1 Methylation 

摘      要：BACKGROUND Gastric cancer(GC)is a malignant tumor originating from gastric mucosal epithelial cells that has high morbidity and ***(miR)are important diagnostic markers and therapeutic targets in this *** To explore the mechanism of miR-125a-5p in the pathogenesis of *** The expression levels of miR-125a-5p,SERPINE1 and DNMT1 in GC cells and tissues were detected by real-time polymerase chain reaction(PCR)and Western ***-specific PCR was used to detect the level of miR-125a-5p methylation.A cell counting kit 8 assay,scratch test,and a Transwell assay were performed to detect the proliferation,migration,and invasiveness of HGC27 cells,*** expression of the epithelial mesenchymal transition(EMT)-related proteins E-cadherin,N-cadherin and vimentin in HGC27 cells was detected by Western blotting,while the expression of vimentin was detected by *** This study revealed that miR-125a-5p was expressed at low levels in GC clinical samples and cells and that miR-125a-5p overexpression inhibited the proliferation,migration,invasiveness and EMT of GC ***,miR-125a-5p can reduce GC cell proliferation,promote E-cadherin expression,inhibit N-cadherin and vimentin expression,and reduce the EMT of GC cells,thus constraining GC cells to a certain ***,DNMT1 inhibited miR-125a-5p expression by increasing the methylation of the miR-125a-5p promoter,thereby promoting the expression of SERPINE1,which acts together with miR-125a-5p to exert antagonistic effects on *** Our study revealed that DNMT1 promoted SERPINE1 protein expression by inducing miR-125a-5p methylation,which led to the proliferation,migration and occurrence of EMT in GC cells.