Genome-Wide Identification and Expression Analysis of Calmodulin-Like Proteins in Tobacco

作     者：Mengjie Xu Anbin Wang Tonghong Zuo Hecui Zhang Zhihao Hu Liquan Zhu 

作者机构：College of Agronomy and BiotechnologySouthwest UniversityChongqing400715China 

出 版 物：《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志(英文))

年 卷 期：2025年第94卷第1期

页      面：157-179页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：College of Agronomy and Biotechnology Southwest University(4412200577) 

主　　题：Calmodulin-like(CMLs) tobacco plant growth abiotic stress 

摘      要：Calmodulin-like (CMLs) proteins are critical in calcium signaling and essential for plant growth, development, and stress responses. In many species, the CMLs families have been identified and described. However, the characterization and expression profiling of CMLs genes in tobacco is retrievable. In this study, a comprehensive whole-genome identification and analysis, and 75 NtCML genes were identified in tobacco, each containing two to four EF-hand domains. Most NtCML proteins exhibited conserved gene structures and motifs. Notably, most NtCML proteins were intron-less and distributed across 18 chromosomes. Two pairs of tandemly duplicated genes and seven pairs of segmentally duplicated genes were identified within the tobacco genome. Furthermore, 22 pairs of orthologous CMLs genes were discovered between Arabidopsis and tobacco. Cis-acting element analysis revealed that elements associated with hormones, stress responses, and plant growth and development were found in the promoter regions. Expression analysis indicated that some NtCML genes displayed tissue-specific expression patterns. Specifically, NtCML12, NtCML18, NtCML27, and NtCML28 showed significant upregulation during cold acclimation treatment. These results indicate that tobacco CMLs act as Ca^(2+) signal transducers, regulating plant growth and abiotic stress responses.