HPLC–MS and HPLC–MS/MS analysis of seven active constituents of Xiao-Xu-Ming decoction and application to a pharmacokinetic study after oral administration to rat

作     者：Yilin Wang Chunguang Ding Caisheng Wu Kehe Du Jinlan Zhangn Hailin Qin Jinfeng Hou Guanhua Du 

作者机构：State Key Laboratory of Bioactive Substances and Functions of Natural MedicinesInstitute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China 

出 版 物：《Acta Pharmaceutica Sinica B》 (药学学报（英文版）)

年 卷 期：2012年第2卷第2期

页      面：188-197页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：the National Natural Science Foundation of China(No.30630073) the Innovation Method Fund from Ministry of Science and Technology of China(2009IM031600)for financial support 

主　　题：Xiao-Xu-Ming decoction HPLC-MS HPLC-MS/MS Pharmacokinetics Oroxylin A-7-O-glucuronide Wogonoside Liquiritigenin Cimifugin 5-O-methylvisammiol Glycyrrhizic acid Glycyrrhetinic acid 

摘      要：Xiao-xu-ming decoction(XXMD)is a traditional Chinese medicine that has been widely used to treat theoplegia and its *** paper reports the development of three separate assays based on reversed phase high-performance liquid chromatography–mass spectrometry(HPLC–MS)and HPLC–MS/MS for the determination of seven active constituents of XXMD viz oroxylin A-7-O-glucuronide,wogonoside,liquiritigenin,cimifugin,5-O-methylvisammiol,glycyrrhizic acid and glycyrrhetinic acid in rat *** calibration curves were linear(r 0.99)with lower limits of quantitation(LLOQs)12.4 ng/***-and inter-day precisions(as relative standard deviation)were all 10.7% with recoveries in the range of 88.7–113%.In addition,the seven analytes were shown to be stable in rat plasma samples under relevant storage *** validated methods were successfully applied to a pharmacokinetic study in rat after oral administration of XXMD.