Lipid-albumin nanoassemblies co-loaded with borneol and paclitaxel for intracellular drug delivery to C6 glioma cells with P-gp inhibition and its tumor targeting

作     者：Bo Tang Guihua Fang Ying Gao Yi Liu Jinwen Liu Meijuan Zou Lihong Wang Gang Cheng 

作者机构：School of PharmacyShenyang Pharmaceutical University103Wenhua RoadShenyang 110016China School of Pharmaceutical EngineeringShenyang Pharmaceutical University103Wenhua RoadShenyang 110016China 

出 版 物：《Asian Journal of Pharmaceutical Sciences》 (亚洲药物制剂科学（英文）)

年 卷 期：2015年第10卷第5期

页      面：363-371页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Borneol Paclitaxel Lipid-albumin nanoassemblies C6 glioma cells P-gp inhibition 

摘      要：Successful chemotherapy with paclitaxel(PTX)is impeded by multidrug resistance(MDR)in tumor *** this study,lipid-albumin nanoassemblies co-loaded with borneol and paclitaxel(BOR/PTX LANs)were prepared to circumvent MDR in C6 glioma *** physiochemical properties including particle size,encapsulation efficiency and morphology were evaluated in *** and qualitative investigations of cellular uptake were carried out in C6 glioma *** cytotoxicity of the BOR/PTX LANs was determined by MTT *** that,the tumor targeting was also evaluated in C6 glioma bearing mice by in vivo imaging ***/PTX LANs have a higher entrapment efficiency(90.4±1.2%),small particle size(107.5±3.2 nm),narrow distribution(P.I.=0.171±0.02).The cellular uptake of PTX was significantly increased by BOR/PTX LANs compared with paclitaxel loaded lipidalbumin nanoassemblies(PTX LANs)in quantitative *** result was further confirmed by confocal laser scanning microscopy *** cellular uptake was energy-,timeand concentration-dependent,and clathrin-and endosome/lysosome-associated pathways were *** BOR/PTX LANs displayed a higher cytotoxicity agaist C6 glioma cells in comparion with PTX LANs and ***,the encapsulation of BOR in LANs obviously increased the accumulation of the drug in tumor tissues,demonstrating the tumor targeted ability of BOR/PTX *** results indicated that BOR/PTX LANs could overcome MDR by combination of drug delivery systems and P-gp inhibition,and shown the potential for treatment of gliomas.