Pyridoxal 5'-phosphate alleviates prenatal pyridaben exposureinduced anxiety-like behaviors in offspring

作     者：Xingwang Ding Ya Wen Xuan Ma Yuepei Zhang Yuting Cheng Zhaofeng Liu Weiyue Hu Yankai Xia 

作者机构：State Key Laboratory of Reproductive MedicineCenter for Global HealthSchool of Public HealthNanjing Medical University101 Longmian AvenueJiangning DistrictNanjing211166China Key Laboratory of Modern Toxicology of Ministry of EducationSchool of Public HealthNanjing Medical University101 Longmian AvenueJiangning DistrictNanjing211166China Department of Chronic Non-Communicable Disease ControlWuxi Liangxi District Center for Disease Control and PreventionWuxi214011China Department of Nutrition and Food SafetySchool of Public HealthNanjing Medical UniversityNanjing211166China 

出 版 物：《Environmental Science and Ecotechnology》 (环境科学与生态技术（英文）)

年 卷 期：2023年第13卷第1期

页      面：125-133页

核心收录：

学科分类：1002[医学-临床医学] 100211[医学-妇产科学] 10[医学] 

基　　金：supported by the National Key R&D Program of China(grant number 2021YFC2701100 and 2021YFC2701102) China-U.S.Program for Biomedical Collaborative Research(NSFCNIH)(grant number 81961128022) the National Natural Science Foundation of China(grant number 81903351) the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD) 

主　　题：Pyridaben Prenatal exposure Pyridoxal 50-phosphate Anxiety-like behaviors 

摘      要：Pyridaben(PY)is a widely used organochlorine acaricide,which can be detected in the peripheral blood of pregnant *** evidence suggests that PY has reproductive ***,it remains uncertain whether prenatal PY exposure impacts neurobehavioral development in ***,we administered PY to pregnant mice at a dose of 0.5 and 5 mg kg^(-1)day^(-1)via gavage and observed anxietylike behaviors in PY offspring aged five *** then integrated the metabolome and transcriptome of the offspring s brain to explore the underlying *** data indicated that the vitamin B6 metabolism pathway was significantly affected,and the pyridoxal 50-phosphate(PLP)concentration and the active form of vitamin B6 was significantly ***,the transcriptome data showed that both PLP generation-related Pdxk and anxiety-related Gad1 were significantly ***,there was a decreasing trend in the concentration of GABA in the hippocampal DG ***,we supplemented PLP at a dose of 20 mg kg^(-1)day^(-1)to the PY offspring via intraperitoneal injection at three *** found up-regulated expression of Pdxk and Gad1 and restored anxiety-like *** study suggests that prenatal exposure to PY can disrupt vitamin B6 metabolism,reduce the concentration of PLP,down-regulate the expression levels of Pdxk and Gad1,inhibit the production of GABA,and ultimately lead to anxiety-like behaviors in offspring.