Effect of SP-A/B in lipoic acid on acute paraquat poisoning

作     者：Shou-peng Li Ji-yuan Han Peng Sun Guo-yan Wu Xiang-yan Bai 

作者机构：Department of Emergency Medicine Union Hospital Tongji Medical College Huazhong University of Science and Technology 

出 版 物：《World Journal of Emergency Medicine》 (世界急诊医学杂志（英文）)

年 卷 期：2014年第5卷第1期

页      面：57-62页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by a grant from the National Natural Science Foundation project of China(30671783) 

主　　题：Lipoic acid Paraquat Acute lung injury Surfactant protein Tumor necrosis factor-α Oxygen free radical Superoxide dimutase Malonaldehyde 

摘      要：BACKGROUND: This study was undertaken to observe the concentration of SP-A/B and the pulmonary surfactant in the lung tissue of rats with acute lung injury/acute respiratory distress syndrome caused by paraquat poisoning after the treatment of metabolic antioxidant-lipoic acid and whether its influence was related to TNF-α.METHODS: Sixty-six male Sprage-Dawley rats were randomly divided into three groups: normal control group(NS group), 6 rats; paraquat poisoning group(PQ group), 30 rats; and paraquat+lipoic acid treatment group(LA group), 30 rats. The rats in the PQ and LA groups were subdivided into 3-, 6-, 12-, 24-, 48-hour subgroups, with 6 rats in each group. After the rats were sacrificed, lung tissue from the same part was taken from the rats. After HE staining, histological changes were observed in the tissue under a light microscope. Lung tissue was also taken to test the levels of superoxide dismutase(SOD) and malondialdehyde(MDA). Whole blood(0.8 mL) without anticoagulant was drawn from the tail vein of rats for the determination of the TNF-α level. The total RNA of the lung tissue was collected, and the Rt-PCR method was used to measure the levels of SP-A and SP-B ***: HE staining showed that histopathological changes were milder in the LA group than in the PQ group. There were significant differences in MDA and SOD levels between different intervals both in intergroups and intragroups except the 3-hour subgroup(P0.01). Likewise, the significant differences in the levels of TNF-α were also present between the three groups and between different intervals(P0.01). The significant differences in SP-A mRNA and SP-B mRNA amplification ratio were seen between the three groups at the same intervals(P0.01), but the differences between different intervals in the PQ group were statistically significant(P0.05). The differences between different intervals in the LA group were statistically significant(P0.01).CONCLUSION: Lipoic acid in acute paraquat poi