Fourier analysis of elastic light scattering spectrum of epithelial cell nuclei

作     者：王清华 李振华 来建成 贺安之 Qinghua Wang;Zhenhua Li;Jiancheng Lai;Anzhi He

作者机构：Department of Information Physics and EngineeringNanjing University of Science and Technology 

出 版 物：《Chinese Optics Letters》 (中国光学快报（英文版）)

年 卷 期：2010年第8卷第3期

页      面：278-281页

核心收录：

学科分类：070207[理学-光学] 07[理学] 08[工学] 070302[理学-分析化学] 0703[理学-化学] 0803[工学-光学工程] 0702[理学-物理学] 

基　　金：supported by the National Natural Science Foundation of China(Nos.10904072 and 10704039) the Startup Foundation(No.AB411994) the Development Foundation from Nanjing University of Science and Technology(No.XKF09043) 

主　　题：Fast Fourier transforms Fourier analysis Light refraction Light scattering Refraction Refractive index Refractometers 

摘      要：A new method for simultaneously determining the size and refractive index of epithelial cell nuclei is presented. The function of the modified elastic light scattering spectrum is regarded as a function of wave number factor, Q=2λ-1sin（θ/2）. The modified spectrum has a constant oscillation period with its frequency proportional to the average diameter of cell nuclei. To the same average diameter, the different relative refractive indexes of epithelial cell nuclei only induce the horizontal shift of the spectra. Both the oscillation frequency and the horizontal shift are quantified by the fast Fourier transform on the modified spectra. The average diameter can be figured out through the peak frequency divided by the value of the refractive index of the surrounding medium. The phase angle of the peak frequency has an approximate linear relationship with the relative refractive index of epithelial cell nuclei.